CACYBP Monoclonal antibody, PBS Only

CACYBP Monoclonal Antibody for WB, IP, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IP, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C2G11

Cat no : 68161-1-PBS

Synonyms

CACYBP, calcyclin binding protein, GIG5, hCacyBP, PNAS 107, RP1 102G20.6, S100A6 binding protein, S100A6BP, Siah interacting protein, SIP

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Validation Data Gallery

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  WB analysis using 68161-1-Ig (same clone as 68161-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 68161-1-Ig (same clone as 68161-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 68161-1-Ig (CACYBP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68161-1-Ig (same clone as 68161-1-PBS)

  IP result of anti-CACYBP (IP:68161-1-Ig, 5ug; Detection:68161-1-Ig 1:10000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68161-1-Ig (same clone as 68161-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CACYBP antibody (68161-1-Ig, Clone: 3C2G11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68161-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68161-1-PBS targets CACYBP in WB, IP, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CACYBP fusion protein Ag2340
• Full Name: calcyclin binding protein
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC022352
• Gene Symbol: CACYBP
• Gene ID (NCBI): 27101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).