CACYBP Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

CACYBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11745-1-PBS

Synonyms

Calcyclin, S100 alpha 6, PNAS 107, hCacyBP, GIG5

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Validation Data Gallery

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  WB analysis of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

  HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue, and rat liver tissue were subjected to SDS PAGE followed by western blot with 11745-1-AP (CACYBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11745-1-AP (same clone as 11745-1-PBS)

  IP result of anti-CACYBP (IP:11745-1-AP, 4ug; Detection:11745-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11745-1-AP (same clone as 11745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11745-1-AP (same clone as 11745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11745-1-AP (CACYBP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CACYBP antibody (11745-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 11745-1-AP (same clone as 11745-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CACYBP Polyclonal antibody (11745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11745-1-PBS in a different storage buffer formulation.

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Product Information

11745-1-PBS targets CACYBP in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CACYBP fusion protein Ag2340
• Full Name: calcyclin binding protein
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC022352
• Gene Symbol: CACYBP
• Gene ID (NCBI): 27101
• RRID: AB_2070266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CACYBP(Calcyclin-binding protein) is also named as S100A6BP, SIP. It is a new target 30 kDa protein that can interact with S100 proteins, including S100A6, S100A1, S100B, and S100P, but not to S100A4, calbindin D, parvalbumin or calmodulin(PMID:22295074). It may be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins and probably serves as a molecular bridge in ubiquitin E3 complexe. It has 2 isoforms produced by alternative splicing and can exist as a dimer(PMID:17916393).