CACTIN Polyclonal antibody

• 

  WB analysis using 32066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32066-1-AP (CACTIN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CACTIN Polyclonal Antibody for WB, ELISA

Cat No. 32066-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Splicing factor Cactin, Renal carcinoma antigen NY-REN-24

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32066-1-AP targets CACTIN in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CACTIN fusion protein Ag35645
• Full Name: chromosome 19 open reading frame 29
• Calculated Molecular Weight: 108 kDa, 89 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: NM_021231
• Gene Symbol: C19orf29
• Gene ID (NCBI): 58509
• RRID: AB_3670183
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8WUQ7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CACTIN plays a role in pre-mRNA splicing by facilitating excision of a subset of introns (PubMed:28062851).CACTIN was reported to be involved in the regulation of innate immune response (PubMed:20829348). CACTIN acts as a negative regulator of Toll-like receptor, interferon-regulatory factor (IRF) and canonical NF-kappa-B signaling pathways (PubMed:20829348, PubMed:26363554). CACTIN contributes to the regulation of transcriptional activation of NF-kappa-B target genes in response to endogenous pro-inflammatory stimuli (PubMed:20829348, PubMed:26363554). CACTIN has two isforms (89kDa and 108 kDa).