CACNG1 Polyclonal antibody

CACNG1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18389-1-AP

Synonyms

Voltage-dependent calcium channel gamma-1 subunit, Dihydropyridine-sensitive L-type, skeletal muscle calcium channel subunit gamma, CACNLG

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Validation Data Gallery

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  IHC staining of human liver cancer using 18389-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18389-1-AP (CACNG1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse skeletal muscle using 18389-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18389-1-AP (CACNG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 18389-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 18389-1-AP (CACNG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 18389-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18389-1-AP (CACNG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18389-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18389-1-AP (CACNG1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat skeletal muscle using 18389-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 18389-1-AP (CACNG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 18389-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 18389-1-AP (CACNG1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HepG2 using 18389-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CACNG1 antibody (18389-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive IHC detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18389-1-AP targets CACNG1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CACNG1 fusion protein Ag12918
• Full Name: calcium channel, voltage-dependent, gamma subunit 1
• Calculated Molecular Weight: 222 aa, 25 kDa
• GenBank Accession Number: BC069493
• Gene Symbol: CACNG1
• Gene ID (NCBI): 786
• RRID: AB_2878540
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.