CACNB4 Polyclonal antibody

CACNB4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17770-1-AP

Synonyms

CAB4, CACNB4, CACNLB4, EA5, EJM

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Validation Data Gallery

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  WB analysis using 17770-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17770-1-AP (CACNB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17770-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17770-1-AP (CACNB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17770-1-AP (CACNB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17770-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17770-1-AP (CACNB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 17770-1-AP is CACNB4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CACNB4 fusion protein Ag12178
• Full Name: calcium channel, voltage-dependent, beta 4 subunit
• Calculated Molecular Weight: 520 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC075049
• Gene Symbol: CACNB4
• Gene ID (NCBI): 785
• RRID: AB_2878438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p.; Authors - Cui Ma