CACNA2D1 Polyclonal antibody

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  WB analysis of mouse brain using 27453-1-AP

  mouse brain tissue and mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 27453-1-AP (CACNA2D1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 27453-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27453-1-AP (CACNA2D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 27453-1-AP

  pig brain tissue were subjected to SDS PAGE followed by western blot with 27453-1-AP (CACNA2D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 27453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skeletal muscle using 27453-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 27453-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 27453-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27453-1-AP (CACNA2D1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 27453-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CACNA2D1 antibody (27453-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 27453-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using CACNA2D1 antibody (27453-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CACNA2D1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 27453-1-AP

Publications(8)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF-P, CoIP, ELISA

Synonyms

CACNA2, CACNA2D1, CACNL2A, CCHL2A, MHS3

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Tested Applications

• Positive WB detected in: mouse brain tissue, pig brain tissue, rat brain tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

27453-1-AP targets CACNA2D1 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CACNA2D1 fusion protein Ag26190
• Full Name: calcium channel, voltage-dependent, alpha 2/delta subunit 1
• Calculated Molecular Weight: 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: NM_000722
• Gene Symbol: CACNA2D1
• Gene ID (NCBI): 781
• RRID: AB_2880874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P54289
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	IF,WB	Brain Behav Immun; Microglial IL-10 and β-endorphin expression mediates gabapentinoids antineuropathic pain.; Authors - Khalil Ali Ahmad
rat	WB,IF	Heliyon; Cherry leaf decoction inhibits NMDAR expression and thereby ameliorates CUMS- induced depression-like behaviors through downregulation of α2δ-1; Authors - Chuan Jiang
mouse	WB,IHC	iScience; Exercise training affects calcium ion transport by downregulating the CACNA2D1 protein to reduce hypertension-induced myocardial injury in mice; Authors - Shan Gao
human	WB,IHC	J Gastroenterol; CACNA2D1 regulates the progression and influences the microenvironment of colon cancer; Authors - Hiroyuki Inoue ; KD Validated
mouse	WB,CoIP	Neurochem Res; Scavenger Receptor Class B Type I Modulates Epileptic Seizures and Receptor α2δ-1 Expression; Authors - Yunyi Huang
human	WB	J Proteome Res; ChIP-seq and RNA-seq Reveal the Involvement of Histone Lactylation Modification in Gestational Diabetes Mellitus; Authors - Xiaman Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christian (Verified Customer) (11-19-2018)

1:50 - labelled neurons of interest1:200 - labelled 3-4 neurons of interest1:1000 - labelled 1-2 neurons of interest

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:50, 1:200, 1:1000
• Cell Tissue Type: Mouse Spinal Cord/Neurons

CACNA2D1 Antibody Immunohistochemistry,Immunofluorescence, validation (1:50, 1:200, 1:1000 dilution) in Mouse Spinal Cord/Neurons (Cat no:27453-1-AP)