CACHD1 Recombinant antibody

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  WB analysis using 83350-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83350-4-RR (CACHD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83350-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83350-4-RR against Human CACHD1 were performed. The affinity constant is 6.83 nM.

CACHD1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83350-4-RR

Clone No.240197A2

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

VWCD1, RP4 655E10.1, KIAA1573, Cache domain-containing protein 1, cache domain containing 1

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83350-4-RR targets CACHD1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CACHD1 fusion protein Ag22138
• Full Name: cache domain containing 1
• Calculated Molecular Weight: 1274 aa, 142 kDa
• Observed Molecular Weight: 140-170 kDa
• GenBank Accession Number: BC039301
• Gene Symbol: CACHD1
• Gene ID (NCBI): 57685
• RRID: AB_3671008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q5VU97
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CACHD1 (Cache domain-containing protein 1), also called VWCD1 (VWFA and cache domain-containing protein 1), is a type I membrane protein involved that modulates the activities of voltage-gated Ca(2+) channels (VGCCs) (PMID: 30181139; 30404013). CACHD1 has two isoforms, with molecular weights of 142 kDa and 109 kDa respectively. It has certain glycosylation modifications, and the molecular weight of isoform1 after glycosylation modification is 170 kDa (PMID: 30983497; 30404013). This antibody recognizes 142-170 kDa.