Carbonic Anhydrase IX/CA9 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP01136-1

  Sandwich ELISA standard curve of MP01136-1, Human CA9 Recombinant Matched Antibody Pair - PBS only. 84233-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag36941. 84233-5-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP01136-1

  HT-29 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human CA9, and measured 2,951.1 pg/mL.

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  Sample test of MP01136-1

  Urine of six individual healthy human donors was measured. Five measured less than the lowest human CA9 standard, 15.6 pg/mL. One sample read 47.2 pg/mL.

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  Sample test of MP01136-1

  Serum of six individual healthy human donors was measured. The CA9 concentration of detected samples was determined to be 246.1 pg/mL with a range of 71.5-420.1 pg/mL.

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  Cytometric bead array standard curve of MP01136-1

  Cytometric bead array standard curve of MP01136-1, CA9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84233-3-PBS. Detection antibody: 84233-5-PBS. Standard: Ag36941. Range: 0.313-20 ng/mL.

Carbonic Anhydrase IX/CA9 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84233-3-PBS

Clone No.241573G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CAIX, CA9, carbonic anhydrase IX, CA-IX, CA IX

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Product Information

84233-3-PBS targets Carbonic Anhydrase IX/CA9 as part of a matched antibody pair:

MP01136-1: 84233-3-PBS capture and 84233-5-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Carbonic Anhydrase IX/CA9 fusion protein Ag36941
• Full Name: carbonic anhydrase IX
• Calculated Molecular Weight: 459 aa, 50 kDa
• GenBank Accession Number: BC014950
• Gene Symbol: CA9
• Gene ID (NCBI): 768
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16790
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.