CA9 Monoclonal antibody

CA9 Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a


human, mouse, pig, rabbit







Cat no : 66243-1-Ig


CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250

Tested Applications

Positive WB detected inU-87 MG cells, pig stomach tissue, pig brain tissue
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66243-1-Ig targets CA9 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rabbit samples.

Tested Reactivity human, mouse, pig, rabbit
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CA9 fusion protein Ag1540
Full Name carbonic anhydrase IX
Calculated Molecular Weight 459 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC014950
Gene Symbol CA9
Gene ID (NCBI) 768
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.It was observed the 54KDa and 58 Kda bands in A549.


Product Specific Protocols
WB protocol for CA9 antibody 66243-1-IgDownload protocol
IHC protocol for CA9 antibody 66243-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Oncol

Identification and validation of a combined hypoxia and immune index for triple-negative breast cancer.

Authors - Shaoquan Zheng


Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression

Authors - Qiangqiang Liu

Cell Rep

ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis

Authors - Fangquan Chen

Int J Gen Med

Identification of Hypoxia-Associated Signature in Colon Cancer to Assess Tumor Immune Microenvironment and Predict Prognosis Based on 14 Hypoxia-Associated Genes

Authors - Peng Chen

J Transl Med

Tumor microenvironment in a minipig model of spinal cord glioma

Authors - Muhibullah S Tora


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Daniel (Verified Customer) (04-16-2019)

Specific band in hypoxic mouse kidney.Note: The lower part of the blot (<25 kDa) was stained for a hypoxic marker (Ndufa4l2).

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse kidney, hypoxic (lane1), normoxic (lane2)
CA9 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, hypoxic (lane1), normoxic (lane2) (Cat no:66243-1-Ig)