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CA9 Polyclonal antibody
CA9 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 11071-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, mouse brain tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | normal stomach, human renal cell carcinoma tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 21 publications below |
| IHC | See 24 publications below |
| IF | See 7 publications below |
Product Information
11071-1-AP targets CA9 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CA9 fusion protein Ag1540 |
| Full Name | carbonic anhydrase IX |
| Calculated Molecular Weight | 459 aa, 50 kDa |
| Observed Molecular Weight | 60-70 kDa |
| GenBank Accession Number | BC014950 |
| Gene Symbol | CA9 |
| Gene ID (NCBI) | 768 |
| RRID | AB_2066528 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular. Western blotting detected CA9 protein at a molecular weight of 70 kDa (PMID: 31819036).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CA9 antibody 11071-1-AP | Download protocol |
| IHC protocol for CA9 antibody 11071-1-AP | Download protocol |
| IP protocol for CA9 antibody 11071-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A CRLX101 nanoparticles localize in human tumors and not in adjacent, nonneoplastic tissue after intravenous dosing. | ||
Biomed Pharmacother Para-toluenesulfonamide, a novel potent carbonic anhydrase inhibitor, improves hypoxia-induced metastatic breast cancer cell viability and prevents resistance to αPD-1 therapy in triple-negative breast cancer | ||
Oxid Med Cell Longev Carbonic Anhydrase IX Controls Vulnerability to Ferroptosis in Gefitinib-Resistant Lung Cancer | ||
Oxid Med Cell Longev Targeting the Ang2/Tie2 Axis with Tanshinone IIA Elicits Vascular Normalization in Ischemic Injury and Colon Cancer. | ||
Eur J Nucl Med Mol Imaging In vivo three-dimensional evaluation of tumour hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT. | ||
J Invest Dermatol Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Josh (Verified Customer) (10-28-2021) | 15 ug of human clear cell renal cell carcinoma cell line RCC4 was resolved on 15% Tris/Glycine and transferred to PVDF membrane. Membrane was blocked in blocking buffer (2% BSA in TBS/0.1% Tween-20) for 1hr at room temperature, followed by overnight incubation with anti-CA9 (1:1000) in blocking buffer at 4oC. After 1hr incubation with anti-Rabbit secondary antibody, membrane was imaged with ECL. Expected band was detected along with several non-specific bands by western blot
 |
FH K (Verified Customer) (08-03-2021) | total cell lysate (50 ug of human HCC cell lines) was resolved on 10% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in TBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-CA9 in blocking buffer (1:500) at 40C overnight. After 1h incubation with appropriate secondary antibody (anti-Rabbit 1:5000) membranes were imaged with ECL and expected band was detected.
 |
FH Paulo (Verified Customer) (03-04-2019) | Total cell lysate (50 ug of human ccRCC cell lines) was resolved on 10% Bis-Tris gel and transferred to PVDF membrane. Membrane was incubated in blocking buffer (5% milk in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-CAIX in blocking buffer (1:250) at 4C overnight. After 1h incubation with appropriate secondary antibody (DAKO anti-Rabbit 1:10000) membranes were imaged with ECL and expected band was detected
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