CA7 Polyclonal antibody

CA7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13670-1-AP

Synonyms

CA-VII, CAVII, carbonic anhydrase VII, Carbonic anhydrase 7, Carbonate dehydratase VII

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Validation Data Gallery

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  WB analysis using 13670-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13670-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13670-1-AP (CA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13670-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13670-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13670-1-AP (CA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 13670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 13670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 13670-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 13670-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13670-1-AP (CA7 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 13670-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13670-1-AP (CA7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human liver cancer tissue, human kidney tissue, human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13670-1-AP targets CA7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CA7 fusion protein Ag4649
• Full Name: carbonic anhydrase VII
• Calculated Molecular Weight: 264 aa, 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC033865
• Gene Symbol: CA7
• Gene ID (NCBI): 766
• RRID: AB_2243629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse,rat,pig	IF	Cell Regen; Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium.; Authors - Haonan Li