CA5B Polyclonal antibody

CA5B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13342-1-AP

Synonyms

CA VB, CA5B, Carbonate dehydratase VB, Carbonic anhydrase VB

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Validation Data Gallery

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  WB analysis of mouse testis using 13342-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13342-1-AP (CA5B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13342-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13342-1-AP (CA5B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 13342-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13342-1-AP (CA5B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13342-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13342-1-AP (CA5B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13342-1-AP targets CA5B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CA5B fusion protein Ag4166
• Full Name: carbonic anhydrase VB, mitochondrial
• Calculated Molecular Weight: 317 aa, 36 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC028142
• Gene Symbol: CA5B
• Gene ID (NCBI): 11238
• RRID: AB_2243612
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA5B(Carbonic anhydrase 5B, mitochondrial) is also named as CA-VB and belongs to the alpha-carbonic anhydrase family.CA5B encodes a 317-amino acid protein that has 64% sequence similarity with CA5 (114761), a mitochondrial CA, and contains a 33-amino-acid residue signal sequence. Western blot analysis revealed a 36-kD protein precursor and a 32-kD mature protein(PMID:10409679).

Publications

Species	Application	Title
human	WB	J Biol Chem; Non-redundant roles of DIAPHs in primary ciliogenesis.; Authors - Oliva Palander