CA2 Polyclonal antibody

CA2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (2)

Cat no : 16961-1-AP

Synonyms

CA II, CA2, CAC, CAII, Car2, Carbonate dehydratase II, Carbonic anhydrase 2, Carbonic anhydrase C, carbonic anhydrase II

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Validation Data Gallery

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  WB analysis using 16961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 16961-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16961-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 16961-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 16961-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 16961-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 16961-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 16961-1-AP

  IP result of anti-CA2 (IP:16961-1-AP, 4ug; Detection:16961-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, human stomach tissue, mouse colon tissue, mouse kidney tissue, mouse ovary tissue, rat kidney tissue, U2OS cells
• Positive IP detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 3 publications below

Product Information

16961-1-AP targets CA2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CA2 fusion protein Ag10680
• Full Name: carbonic anhydrase II
• Calculated Molecular Weight: 260 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC011949
• Gene Symbol: CA2
• Gene ID (NCBI): 760
• RRID: AB_2065857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA2(carbonic anhydrase II ) is also named as Car2, CA-II, CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in the development of brain(PMID:16825953). Defects in CA2 are the cause of osteopetrosis autosomal recessive type 3 (OPTB3).

Publications

Species	Application	Title
mouse	WB	Mol Cell Proteomics; Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.; Authors - Khositseth Sookkasem S
human	WB,IHC	Sci Rep; SWATH-based proteomics identified carbonic anhydrase 2 as a potential diagnosis biomarker for nasopharyngeal carcinoma.; Authors - Yanzhang Luo
human	WB	J Inflamm Res; M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs; Authors - Xianqin Song
mouse	WB	Bone; Evidence that pyrophosphate acts as an extracellular signalling molecule to exert direct functional effects in primary cultures of osteoblasts and osteoclasts; Authors - Lucie E Bourne
mouse	WB	Front Mol Neurosci; Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model.; Authors - Kalina Wiatr
human	WB	J Cancer; Down-regulated C4orf19 confers poor prognosis in colon adenocarcinoma identified by gene co-expression network.; Authors - Wanpeng Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Adrianna (Verified Customer) (10-31-2022)

Very good antibody, 1:1000 dilution incubated ON at 4 deg C gave a strong signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7

FH

Isabel (Verified Customer) (07-03-2020)

Antibody was used to detect carbonic anhydrase II in protein samples taken from primary mouse osteoclasts. The blot produced was really clean with no other bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Primary mouse osteoclasts

CA2 Antibody Western Blot validation (1:500 dilution) in Primary mouse osteoclasts (Cat no:16961-1-AP)