CA14 Polyclonal antibody

CA14 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13736-1-AP

Synonyms

CA XIV, CA14, Carbonate dehydratase XIV, Carbonic anhydrase 14, carbonic anhydrase XIV

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Validation Data Gallery

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  WB analysis using 13736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13736-1-AP (CA14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13736-1-AP (CA14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 13736-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 13736-1-AP (CA14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse eye tissue, mouse retina tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13736-1-AP targets CA14 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CA14 fusion protein Ag4673
• Full Name: carbonic anhydrase XIV
• Calculated Molecular Weight: 337 aa, 38 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC034412
• Gene Symbol: CA14
• Gene ID (NCBI): 23632
• RRID: AB_2065836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carbonic anhydrase XIV (CAXIV, Car14) is highly expressed in the hepatocyte with predominance in the canalicular membrane and its active site in the extracellular milieu. The CA XIV isoform may also be of importance for regulation of neuronal excitability and transport processes in brain.

Publications

Species	Application	Title
mouse	WB	Glia; Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome; Authors - Sophie B Siems