CA12 Polyclonal antibody

• Featured Product
• KD/KO Validated

CA12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 15180-1-AP

Synonyms

CA-XII, CAXII, carbonic anhydrase XII, Carbonic anhydrase 12, Carbonate dehydratase XII

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Validation Data Gallery

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  WB analysis of MCF-7 using 15180-1-AP

  WB result of CA12 antibody (15180-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA12 transfected MCF-7 cells.

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  WB analysis using 15180-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 15180-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 15180-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15180-1-AP (CA12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15180-1-AP

  WB result of CA12 antibody (15180-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CA12 transfected A549 cells.

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  IHC staining of human brown disease using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 15180-1-AP (CA12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15180-1-AP (CA12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 15180-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 15180-1-AP (CA12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, BxPC-3 cells, MCF-7 cells
• Positive IHC detected in: human brown disease, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

15180-1-AP targets CA12 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CA12 fusion protein Ag7107
• Full Name: carbonic anhydrase XII
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 44 kDa, 75-80 kDa
• GenBank Accession Number: BC000278
• Gene Symbol: CA12
• Gene ID (NCBI): 771
• RRID: AB_2065826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reverses the hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

Publications

Species	Application	Title
human	WB, IF	J Exp Med; Large pH oscillations promote host defense against human airways infection.; Authors - Dusik Kim
human	WB	Mol Oncol; Mechanism of irradiation-induced mammary cancer metastasis: A role for SAP-dependent Mkl1 signaling.; Authors - Maria B Asparuhova
human	WB,IF	Hum Mol Genet; Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease.; Authors - Melissa Lee
human	IHC	Int J Mol Sci; Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma.; Authors - Zihe Huo
human	WB,IF	J Enzyme Inhib Med Chem; Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5.; Authors - Soyoung Hwang
human	WB	Front Oncol; Integrative Proteo-Genomic Analysis for Recurrent Survival Prognosis in Colon Adenocarcinoma.; Authors - FeiYan Ai