• Featured Product
  • KD/KO Validated

CA12 Polyclonal antibody

CA12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15180-1-AP

Synonyms

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, HsT18816, Tumor antigen HOM RCC 3.1.3



Tested Applications

Positive WB detected inA549 cells, BxPC-3 cells, MCF-7 cells
Positive IHC detected inhuman pancreas tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15180-1-AP targets CA12 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA12 fusion protein Ag7107
Full Name carbonic anhydrase XII
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 44 kDa, 75-80 kDa
GenBank Accession NumberBC000278
Gene Symbol CA12
Gene ID (NCBI) 771
RRIDAB_2065826
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reverses the hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.

Protocols

Product Specific Protocols
WB protocol for CA12 antibody 15180-1-APDownload protocol
IHC protocol for CA12 antibody 15180-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

J Exp Med

Large pH oscillations promote host defense against human airways infection.

Authors - Dusik Kim
humanWB

Mol Oncol

Mechanism of irradiation-induced mammary cancer metastasis: A role for SAP-dependent Mkl1 signaling.

Authors - Maria B Asparuhova
humanWB,IF

Hum Mol Genet

Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease.

Authors - Melissa Lee
humanIHC

Int J Mol Sci

Perfusion-Based Bioreactor Culture and Isothermal Microcalorimetry for Preclinical Drug Testing with the Carbonic Anhydrase Inhibitor SLC-0111 in Patient-Derived Neuroblastoma.

Authors - Zihe Huo
humanWB,IF

J Enzyme Inhib Med Chem

Carbonic anhydrase 12 mutation modulates membrane stability and volume regulation of aquaporin 5.

Authors - Soyoung Hwang
humanWB

Front Oncol

Integrative Proteo-Genomic Analysis for Recurrent Survival Prognosis in Colon Adenocarcinoma.

Authors - FeiYan Ai