CA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CA1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (1)

Cat no : 13198-2-AP

Synonyms

Carbonic anhydrase 1, Carbonate dehydratase I, Car1, CA-I, CAB

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Validation Data Gallery

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  WB analysis using 13198-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13198-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 13198-2-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13198-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13198-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13198-2-AP

  IP result of anti-CA1 (IP:13198-2-AP, 4ug; Detection:13198-2-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human breast cancer using 13198-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13198-2-AP (CA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13198-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13198-2-AP (CA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13198-2-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13198-2-AP (CA1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, Jurkat cells, rat spleen tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

13198-2-AP targets CA1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CA1 fusion protein Ag3932
• Full Name: carbonic anhydrase I
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC027890
• Gene Symbol: CA1
• Gene ID (NCBI): 759
• RRID: AB_2275041
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CA1(Carbonic anhydrase 1) is also named as CAB and belongs to the alpha-carbonic anhydrase family, which may reside in cytoplasm, in mitochondria, or in secretory granules, or associate with membranes in cell. It forms a large family of genes encoding zinc metalloenzymes of great physiologic importance. Extracellular CA1 mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation(PMID:17259996).

Publications

Species	Application	Title
mouse,rat	WB,IF	Acta Neuropathol Commun; Upregulation of carbonic anhydrase 1 beneficial for depressive disorder; Authors - Ke Cheng ; KO Validated
rat	WB	J Ginseng Res; Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway; Authors - Mei-Li Lu
mouse	IF	FASEB J; Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.; Authors - Brittany R Jenkins
human	WB	J Inflamm Res; M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs; Authors - Xianqin Song
mouse	WB	Am J Physiol Lung Cell Mol Physiol; Carbonic anhydrase and soluble adenylate cyclase regulation of cystic fibrosis cellular phenotypes.; Authors - Kathleen Boyne
human,rat	WB	Front Pharmacol; Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.; Authors - Meili Lu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Brittany (Verified Customer) (09-27-2019)

Using the CA-1 primary antibody with mouse colon scrapes yielded very clean bands with western blotting. Immunofluorescence also gave a fairly clean, detectable signal, with CA-1 showing up along the brush border in mouse colon tissue. There were some cells that stained more intracellularly, and unsure if that was non-specific binding of the antibody, or another cell type other than colonocytes that expresses CA-1 intracellularly.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 (WB) and 1:100 (IF)
• Cell Tissue Type: Colon tissue (scrapes for western blot)

CA1 Antibody Western Blot,Immunofluorescence, validation (1:1000 (WB) and 1:100 (IF) dilution) in Colon tissue (scrapes for western blot) (Cat no:13198-2-AP)