C9orf89 Recombinant antibody

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  WB analysis using 85357-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85357-1-RR (C9orf89 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 85357-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C9orf89 antibody (85357-1-RR, Clone: 242638F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85357-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85357-1-RR against Human C9orf89 were performed. The affinity constant is 0.756 nM.

C9orf89 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85357-1-RR

Clone No.242638F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Caspase recruitment domain-containing protein 19, CARD19, BinCARD, Bcl10-interacting CARD protein, 242638F7

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, C2C12 cells, H9C2 cells, rat kidney tissue, mouse kidney tissue, human placenta tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85357-1-RR targets C9orf89 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: C9orf89 fusion protein Ag14084
• Full Name: chromosome 9 open reading frame 89
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 15-21 kDa
• GenBank Accession Number: BC004500
• Gene Symbol: C9orf89
• Gene ID (NCBI): 84270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96LW7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C9orf89 (also known as CARD19 or BinCARD) is a mitochondria-localized protein that inhibits BCL10-induced NF-κB activation mainly through its caspase recruitment structural domain (CARD). It plays a role in T cell receptor (TCR) signaling, but deletion of endogenous CARD19 has a lesser effect on Bcl10-dependent NF-κB activation. In addition, C9orf89 is expressed in multiple cell types and is involved in the regulation of inflammatory and immune responses.