C9orf89 Polyclonal antibody

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  WB analysis using 20160-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20160-1-AP (C9orf89 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

C9orf89 Polyclonal Antibody for WB, ELISA

Cat No. 20160-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Caspase recruitment domain-containing protein 19, CARD19, BinCARD, Bcl10-interacting CARD protein

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Tested Applications

• Positive WB detected in: A549 cells, C2C12 cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20160-1-AP targets C9orf89 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C9orf89 fusion protein Ag14084
• Full Name: chromosome 9 open reading frame 89
• Calculated Molecular Weight: 228 aa, 26 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC004500
• Gene Symbol: C9orf89
• Gene ID (NCBI): 84270
• RRID: AB_3669326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96LW7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C9orf89 (also known as CARD19 or BinCARD) is a mitochondria-localized protein that inhibits BCL10-induced NF-κB activation mainly through its caspase recruitment structural domain (CARD). It plays a role in T cell receptor (TCR) signaling, but deletion of endogenous CARD19 has a lesser effect on Bcl10-dependent NF-κB activation. In addition, C9orf89 is expressed in multiple cell types and is involved in the regulation of inflammatory and immune responses.