C9orf72 Monoclonal antibody, PBS Only

C9orf72 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F9A2

Cat no : 67824-1-PBS

Synonyms

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 2F9A2

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Validation Data Gallery

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  WB analysis using 67824-1-Ig (same clone as 67824-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 67824-1-Ig (same clone as 67824-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 67824-1-Ig (same clone as 67824-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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  WB analysis of C6 using 67824-1-Ig (same clone as 67824-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67824-1-Ig (same clone as 67824-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67824-1-Ig (same clone as 67824-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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  WB analysis of U-87 MG using 67824-1-Ig (same clone as 67824-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67824-1-Ig (same clone as 67824-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67824-1-Ig (same clone as 67824-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 67824-1-Ig (same clone as 67824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 67824-1-Ig (same clone as 67824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse intestine using 67824-1-Ig (same clone as 67824-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293T using 67824-1-Ig (same clone as 67824-1-PBS)

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human C9orf72 (67824-1-Ig, Clone:2F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

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Product Information

67824-1-PBS targets C9orf72 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: C9orf72 fusion protein Ag21080
• Full Name: chromosome 9 open reading frame 72
• Calculated Molecular Weight: 481 aa, 54 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC020851
• Gene Symbol: C9orf72
• Gene ID (NCBI): 203228
• RRID: AB_2918586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.