C7 Recombinant antibody

C7 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241551D6

Cat no : 84263-5-RR

Synonyms

Complement component C7, complement component 7, 241551D6

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Validation Data Gallery

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  WB analysis of human plasma using 84263-5-RR

  human plasma were subjected to SDS PAGE followed by western blot with 84263-5-RR (C7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84263-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84263-5-RR against Human C7 were performed. The affinity constant is 1.22 nM.

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Tested Applications

• Positive WB detected in: human plasma

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84263-5-RR is C7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: complement component 7
• Calculated Molecular Weight: 94kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: NM_000587.4
• Gene Symbol: C7
• Gene ID (NCBI): 730
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C7, a single-chain plasma glycoprotein, is a component of the complement system. It is also a constituent of the membrane attack complex (MAC), which plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C7 serves as a membrane anchor. People with C7 deficiency are prone to bacterial infection.