C6orf182 Polyclonal antibody

C6orf182 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24957-1-AP

Synonyms

Cep57R, CEP57L1, Cep57 related protein, Centrosomal protein of 57 kDa-related protein, Centrosomal protein CEP57L1



Tested Applications

Positive WB detected inA549 cells, mouse kidney tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

24957-1-AP targets C6orf182 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C6orf182 fusion protein Ag21054
Full Name chromosome 6 open reading frame 182
Calculated Molecular Weight 460 aa, 54 kDa
Observed Molecular Weight 55-65 kda
GenBank Accession NumberBC064365
Gene Symbol C6orf182
Gene ID (NCBI) 285753
RRIDAB_2879821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C6orf182 also named as Centrosomal protein of 57 kDa related protein is a 460 amino-acid protein, which belongs to translokin family. C6orf182 as centrosomal protein may be required for microtubule attachment to centrosomes and localizes in the cytoplasm. The function of C6orf182 need to be further studied.

Protocols

Product Specific Protocols
WB protocol for C6orf182 antibody 24957-1-APDownload protocol
IF protocol for C6orf182 antibody 24957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

J Cell Biol

Cep57 and Cep57L1 maintain centriole engagement in interphase to ensure centriole duplication cycle.

Authors - Kei K Ito
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Jay (Verified Customer) (10-03-2019)

-Methanol Fixation- IF result of C6orf182 antibody shows that C6orf182 (Cep57l1) localizes to centrosome in mouse NIH3T3 cell line- C6orf182(red), Centrin(green; centrosome marker) and DAPI(blue;nucleus)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse NIH3T3
C6orf182 Antibody Immunofluorescence, validation (1:100 dilution) in Mouse NIH3T3 (Cat no:24957-1-AP)