C6orf182 Polyclonal antibody

C6orf182 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 24957-1-AP

Synonyms

Cep57R, CEP57L1, Cep57 related protein, Centrosomal protein of 57 kDa-related protein, Centrosomal protein CEP57L1

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Validation Data Gallery

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  WB analysis using 24957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24957-1-AP (C6orf182 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 24957-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C6orf182 antibody (24957-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, mouse kidney tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

24957-1-AP targets C6orf182 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

• Tested Reactivity: human, mouse, monkey
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C6orf182 fusion protein Ag21054
• Full Name: chromosome 6 open reading frame 182
• Calculated Molecular Weight: 460 aa, 54 kDa
• Observed Molecular Weight: 55-65 kda
• GenBank Accession Number: BC064365
• Gene Symbol: C6orf182
• Gene ID (NCBI): 285753
• RRID: AB_2879821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C6orf182 also named as Centrosomal protein of 57 kDa related protein is a 460 amino-acid protein, which belongs to translokin family. C6orf182 as centrosomal protein may be required for microtubule attachment to centrosomes and localizes in the cytoplasm. The function of C6orf182 need to be further studied.

Publications

Species	Application	Title
human	IF	J Cell Biol; Cep57 and Cep57L1 maintain centriole engagement in interphase to ensure centriole duplication cycle.; Authors - Kei K Ito
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jay (Verified Customer) (10-03-2019)

-Methanol Fixation- IF result of C6orf182 antibody shows that C6orf182 (Cep57l1) localizes to centrosome in mouse NIH3T3 cell line- C6orf182(red), Centrin(green; centrosome marker) and DAPI(blue;nucleus)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse NIH3T3

C6orf182 Antibody Immunofluorescence, validation (1:100 dilution) in Mouse NIH3T3 (Cat no:24957-1-AP)