C6orf15 Polyclonal antibody, PBS Only

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  WB analysis using 24953-1-AP (same clone as 24953-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 24953-1-AP (C6orf15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24953-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 24953-1-AP (same clone as 24953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24953-1-AP (C6orf15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24953-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 24953-1-AP (same clone as 24953-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24953-1-AP (C6orf15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24953-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 24953-1-AP (same clone as 24953-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using C6orf15 antibody (24953-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 24953-1-PBS in a different storage buffer formulation.

C6orf15 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24953-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

24953-1-PBS targets C6orf15 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C6orf15 fusion protein Ag20276
• Full Name: chromosome 6 open reading frame 15
• Calculated Molecular Weight: 325 aa, 34 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC153157
• Gene Symbol: C6orf15
• Gene ID (NCBI): 29113
• RRID: AB_2879818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UXA7
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

C6orf15, also named as Protein STG, is a 325 amino acid protein, which as a secreted protein is expressed in skin and tonsils. The calculated molecular weight of C6orf15 is 34 kDa, but we detected a 50-55 kDa protein by western blot. C6orf15 may contains some modified residues.