C6 Polyclonal antibody, PBS Only

C6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17239-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human blood using 17239-1-AP (same clone as 17239-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 17239-1-AP (C6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

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  IP experiment of human plasma using 17239-1-AP (same clone as 17239-1-PBS)

  IP result of anti-C6 (IP:17239-1-AP, 4ug; Detection:17239-1-AP 1:800) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 17239-1-AP (same clone as 17239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 17239-1-AP (same clone as 17239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17239-1-AP (C6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 17239-1-AP (same clone as 17239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17239-1-AP (C6 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 17239-1-AP (same clone as 17239-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17239-1-AP (C6 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17239-1-PBS in a different storage buffer formulation.

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Product Information

17239-1-PBS targets C6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C6 fusion protein Ag11083
• Full Name: complement component 6
• Calculated Molecular Weight: 934 aa, 105 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC035723
• Gene Symbol: C6
• Gene ID (NCBI): 729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The final consequence of complement activation is formation of the membrane attack complex (MAC), a multiprotein assembly that perforates cell membranes, forming transmembrane channels. C6 is 1 of 5 late-acting proteins of complement that participate in assembly and function of the MAC. The deduced C6 protein contains 934 amino acids, including a 21-amino acid N-terminal signal sequence and 2 N-glycosylation sites. Mutations in the C6 gene are associated with complement component-6 deficiency.