C5 Monoclonal antibody, PBS Only

C5 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2G8C9

Cat no : 66634-1-PBS

Synonyms

C5, C5b, C5, C5a, Complement C5, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

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Validation Data Gallery

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  WB analysis of human plasma using 66634-1-Ig (same clone as 66634-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit serum using 66634-1-Ig (same clone as 66634-1-PBS)

  rabbit serum were subjected to SDS PAGE followed by western blot with 66634-1-Ig (C5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using C5 antibody (66634-1-Ig, Clone: 2G8C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66634-1-Ig (same clone as 66634-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human C5 (66634-1-Ig, Clone:2G8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66634-1-PBS in a different storage buffer formulation.

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Product Information

66634-1-PBS targets C5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: C5 fusion protein Ag17882
• Full Name: complement component 5
• Calculated Molecular Weight: 1676 aa, 188 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC113740
• Gene Symbol: C5
• Gene ID (NCBI): 727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.