C4BPB Polyclonal antibody

C4BPB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15837-1-AP

Synonyms

C4BP, C4b-binding protein beta chain, C4b binding protein beta chain

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Validation Data Gallery

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  WB analysis of human plasma using 15837-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 15837-1-AP (C4BPB Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse ovary using 15837-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15837-1-AP (C4BPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15837-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15837-1-AP (C4BPB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15837-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15837-1-AP (C4BPB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15837-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15837-1-AP (C4BPB Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 15837-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 15837-1-AP (C4BPB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human plasma tissue, HeLa cells, mouse ovary tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15837-1-AP targets C4BPB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C4BPB fusion protein Ag8587
• Full Name: complement component 4 binding protein, beta
• Calculated Molecular Weight: 251 aa, 28 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC005378
• Gene Symbol: C4BPB
• Gene ID (NCBI): 725
• RRID: AB_2067035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113). This polyclonal antibody is raised against the C4BP beta chain (C4BPB).

Publications

Species	Application	Title
human	WB	BMC Cancer; The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.; Authors - Ping Zhao
human	WB	World J Surg Oncol; Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma; Authors - Wei Dong