C4BPA Polyclonal antibody

C4BPA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11819-1-AP

Synonyms

C4BP, C4BPA, Proline rich protein, PRP

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Validation Data Gallery

• 

  WB analysis of human blood using 11819-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 11819-1-AP (C4BPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 11819-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 11819-1-AP (C4BPA Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  IHC staining of human liver cancer using 11819-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11819-1-AP (C4BPA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11819-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11819-1-AP (C4BPA Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human blood tissue, human plasma tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11819-1-AP targets C4BPA in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C4BPA fusion protein Ag2387
• Full Name: complement component 4 binding protein, alpha
• Calculated Molecular Weight: 597 aa, 67 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC022312
• Gene Symbol: C4BPA
• Gene ID (NCBI): 722
• RRID: AB_2877796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C4b-binding protein (C4BP) is a large oligomeric glycoprotein, present in plasma at a concentration of approximately 200 mg/L (PMID: 24218259). It is the main inhibitor of the classical complement and lectin pathway. C4BP is composed of two polypeptides (alpha and beta chains), which have molecular weights of 70 and 45 kDa, respectively (PMID: 7561113). This polyclonal antibody is raised against the C4BP alpha chain (C4BPA).

Publications

Species	Application	Title
		Brain; Extracellular vesicle biomarkers for complement dysfunction in schizophrenia; Authors - Ting Xue
human	IHC	Oncotarget; Hepatitis B virus X protein up-regulates C4b-binding protein α through activating transcription factor Sp1 in protection of hepatoma cells from complement attack.; Authors - Guoxing Feng
human	WB	BMC Cancer; The imbalance in the complement system and its possible physiological mechanisms in patients with lung cancer.; Authors - Ping Zhao
human	WB	World J Surg Oncol; Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma; Authors - Wei Dong

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