C3/C3b/C3c Monoclonal antibody, PBS Only

C3/C3b/C3c Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A6B3

Cat no : 66157-1-PBS

Synonyms

C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9

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Validation Data Gallery

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  WB analysis using 66157-1-Ig (same clone as 66157-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human plasma using 66157-1-Ig (same clone as 66157-1-PBS)

  human plasma was subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human blood using 66157-1-Ig (same clone as 66157-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66157-1-Ig (same clone as 66157-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

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Product Information

66157-1-PBS targets C3/C3b/C3c in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: C3/C3b/C3c fusion protein Ag15955
• Full Name: complement component 3
• Calculated Molecular Weight: 1663 aa, 187 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC150179
• Gene Symbol: C3
• Gene ID (NCBI): 718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes the C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.