C2orf79/PTRHD1 Polyclonal antibody

C2orf79/PTRHD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25576-1-AP

Synonyms

PTRHD1, C2orf79, Putative peptidyl-tRNA hydrolase PTRHD1, Peptidyl-tRNA hydrolase domain-containing protein 1, EC:3.1.1.29

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 25576-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25576-1-AP (C2orf79/PTRHD1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 25576-1-AP

  IP result of anti-C2orf79/PTRHD1 (IP:25576-1-AP, 4ug; Detection:25576-1-AP 1:500) with COLO 320 cells lysate 4000ug.

• 

  IHC staining of human colon cancer using 25576-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25576-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 25576-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25576-1-AP (C2orf79/PTRHD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25576-1-AP targets C2orf79/PTRHD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C2orf79/PTRHD1 fusion protein Ag22255
• Full Name: chromosome 2 open reading frame 79
• Calculated Molecular Weight: 140 aa, 16 kDa
• Observed Molecular Weight: 13-16 kDa
• GenBank Accession Number: BC073803
• Gene Symbol: PTRHD1
• Gene ID (NCBI): 391356
• RRID: AB_2880136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.