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  • KD/KO Validated

RtcB-Specific Polyclonal antibody

RtcB-Specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 19809-1-AP


C22orf28, FAAP, focal adhesion associated protein, hematopoietic stem/progenitor cell 117, HSPC117, RtcB, RtcB-Specific

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19809-1-AP targets RtcB-Specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RtcB-Specific fusion protein Ag13836
Full Name chromosome 22 open reading frame 28
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC000151
Gene Symbol C22orf28
Gene ID (NCBI) 51493
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)


Product Specific Protocols
WB protocol for RtcB-Specific antibody 19809-1-APDownload protocol
IHC protocol for RtcB-Specific antibody 19809-1-APDownload protocol
IF protocol for RtcB-Specific antibody 19809-1-APDownload protocol
IP protocol for RtcB-Specific antibody 19809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.

Authors - Yichen Xu

Mol Cell

A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB.

Authors - Yanyan Lu

Nat Commun

Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells.

Authors - Carmen Aguilar

J Biol Chem

The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.

Authors - Irem Unlu
  • KO Validated

Life Sci Alliance

Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs.

Authors - Alexandra Papaioannou

J Immunol

The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1

Authors - Lei Cao