RtcB-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

RtcB-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 19809-1-AP

Synonyms

HSPC117, RTCB, RNA-splicing ligase RtcB homolog, FAAP, EC:6.5.1.8

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Validation Data Gallery

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  WB analysis using 19809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19809-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19809-1-AP (RtcB-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19809-1-AP

  IP result of anti-RtcB-Specific (IP:19809-1-AP, 3ug; Detection:19809-1-AP 1:500) with HeLa cells lysate 3000ug.

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  IP experiment of HeLa using 19809-1-AP

  IP result of anti-RtcB-Specific (IP:19809-1-AP, 4ug; Detection:19809-1-AP 1:4000) with HeLa cells lysate 1800 ug.

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  IHC staining of human lung cancer using 19809-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 19809-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 19809-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 19809-1-AP

  Immunofluorescent analysis of HeLa cells using 19809-1-AP (RtcB-Specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 19809-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RtcB-Specific Polyclonal antibody (19809-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of A431 using 19809-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.25 ug RtcB-Specific Polyclonal antibody (19809-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IP: See 2 publications below

Product Information

19809-1-AP targets RtcB-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RtcB-Specific fusion protein Ag13836
• Full Name: chromosome 22 open reading frame 28
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC000151
• Gene Symbol: RTCB
• Gene ID (NCBI): 51493
• RRID: AB_10695047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)

Publications

Species	Application	Title
human	WB	Cell; ERα is an RNA-binding protein sustaining tumor cell survival and drug resistance.; Authors - Yichen Xu
human	WB,IP	Mol Cell; A Synthetic Biology Approach Identifies the Mammalian UPR RNA Ligase RtcB.; Authors - Yanyan Lu
pig	WB	Nat Commun; Reprogramming of microRNA expression via E2F1 downregulation promotes Salmonella infection both in infected and bystander cells.; Authors - Carmen Aguilar
human	WB	J Biol Chem; The cyclic phosphodiesterase CNP and RNA cyclase RtcA fine-tune noncanonical XBP1 splicing during ER stress.; Authors - Irem Unlu ; KO Validated
human	IP	Life Sci Alliance; Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs.; Authors - Alexandra Papaioannou
human	WB	J Immunol; The RNA-Splicing Ligase RTCB Promotes Influenza A Virus Replication by Suppressing Innate Immunity via Interaction with RNA Helicase DDX1; Authors - Lei Cao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ilham (Verified Customer) (08-06-2024)

It works very well - the AB was diluted 1:2000 in 5% BSA -chemiluminescence blots using the ChemiDoc Imaging System.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000