Tested Applications
Positive WB detected in | PC-3 cells, mouse testis tissue |
Positive IHC detected in | human stomach tissue, human brown disease Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
IHC | See 1 publications below |
Product Information
14888-1-AP targets C1orf116 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | C1orf116 fusion protein Ag6675 Predict reactive species |
Full Name | chromosome 1 open reading frame 116 |
Calculated Molecular Weight | 64 kDa |
Observed Molecular Weight | 64 kDa, 38 kDa |
GenBank Accession Number | BC000765 |
Gene Symbol | C1orf116 |
Gene ID (NCBI) | 79098 |
RRID | AB_2228225 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9BW04 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Specifically androgen-regulated gene protein (SARG), encoded by C1orf116, is a 601-aa protein considered putative androgen-specific receptor. SARG mRNA is highly expressed in prostate and can be up-regulated by androgens, but not by glucocorticoids. Little is known about the function of SARG to date. Two isoforms of SARG are produced by alternative splicing encoding 64 kDa and 38 kDa proteins, and both isoforms can be recognized by this antibody 14888-1-AP.
Protocols
Product Specific Protocols | |
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WB protocol for C1orf116 antibody 14888-1-AP | Download protocol |
IHC protocol for C1orf116 antibody 14888-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Sci Signal Re-epithelialization of cancer cells increases autophagy and DNA damage: Implications for breast cancer dormancy and relapse | ||
Front Oncol The Specifically Androgen-Regulated Gene (SARG) Promotes Papillary Thyroid Carcinoma (PTC) Lymphatic Metastasis Through Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor 3 (VEGFR-3) Axis. |