C1orf116 Polyclonal antibody

C1orf116 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14888-1-AP

Synonyms

Specifically androgen-regulated gene protein, SARG

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Validation Data Gallery

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  WB analysis of PC-3 using 14888-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14888-1-AP (C1orf116 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 14888-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14888-1-AP (C1orf116 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human brown disease using 14888-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 14888-1-AP (C1orf116 antibody) at dilution of 1:160 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 14888-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14888-1-AP (C1orf116 antibody) at dilution of 1:160 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse testis tissue
• Positive IHC detected in: human stomach tissue, human brown disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

14888-1-AP targets C1orf116 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1orf116 fusion protein Ag6675
• Full Name: chromosome 1 open reading frame 116
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa, 38 kDa
• GenBank Accession Number: BC000765
• Gene Symbol: C1orf116
• Gene ID (NCBI): 79098
• RRID: AB_2228225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Specifically androgen-regulated gene protein (SARG), encoded by C1orf116, is a 601-aa protein considered putative androgen-specific receptor. SARG mRNA is highly expressed in prostate and can be up-regulated by androgens, but not by glucocorticoids. Little is known about the function of SARG to date. Two isoforms of SARG are produced by alternative splicing encoding 64 kDa and 38 kDa proteins, and both isoforms can be recognized by this antibody 14888-1-AP.

Publications

Species	Application	Title
human	WB,IHC	Front Oncol; The Specifically Androgen-Regulated Gene (SARG) Promotes Papillary Thyroid Carcinoma (PTC) Lymphatic Metastasis Through Vascular Endothelial Growth Factor C (VEGF-C) and VEGF Receptor 3 (VEGFR-3) Axis.; Authors - Shuai-Jun Xu