Various lysates were subjected to SDS PAGE followed by western blot with 21506-1-AP (C1orf106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 21506-1-AP (C1orf106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21506-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21506-1-AP (C1orf106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21506-1-AP (C1orf106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 21506-1-AP
IP result of anti-C1orf106 (IP:21506-1-AP, 4ug; Detection:21506-1-AP 1:500) with mouse kidney tissue lysate 4800ug.
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mouse kidney tissue, HEK-293 cells, rat kidney tissue
Positive IP detected in
mouse kidney tissue
Positive IHC detected in
mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HEK-293 cells, HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
21506-1-AP targets C1orf106 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
C1orf106 (also known as innate immunity activator, INAVA) has thus far been considered to play a significant role in intestinal inflammatory diseases. C1orf106 is highly expressed in the human intestine and intestinal epithelial cell lines but expressed at low levels in myeloid cells and mouse bone marrow-derived macrophages (PMID: 29420262). Recent studies have also shown that C1orf106 protects against colitis by stabilizing E-cadherin and bridges mucosal barrier function with inflammatory signaling (PMID: 31376015).
Various lysates were subjected to SDS PAGE followed by western blot with 21506-1-AP (C1orf106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21506-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21506-1-AP (C1orf106 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse kidney using 21506-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21506-1-AP (C1orf106 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 21506-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21506-1-AP (C1orf106 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of mouse kidney using 21506-1-AP
IP result of anti-C1orf106 (IP:21506-1-AP, 4ug; Detection:21506-1-AP 1:500) with mouse kidney tissue lysate 4800ug.
IF/ICC Figures
IF Staining of HEK-293 using 21506-1-AP
Immunofluorescent analysis of HEK-293 cells, using C1orf106 antibody 21506-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IF Staining of HeLa using 21506-1-AP
Immunofluorescent analysis of HeLa cells, using C1orf106 antibody 21506-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
C1orf106 Polyclonal antibody
Catalog Number
21506-1-AP
Citations
-
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
