C1S Polyclonal antibody

C1S Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 14554-1-AP

Synonyms

C1 esterase, C1S, Complement C1s subcomponent

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Validation Data Gallery

• 

  WB analysis of human plasma using 14554-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 14554-1-AP (C1S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14554-1-AP

  rat plasma lysates were subjected to SDS PAGE followed by western blot with 14554-1-AP (C1S antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human plasma using 14554-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 14554-1-AP (C1S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 14554-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14554-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14554-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 14554-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14554-1-AP (C1S antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat plasma, human plasma
• Positive IHC detected in: human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

14554-1-AP targets C1S in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1S fusion protein Ag5977
• Full Name: complement component 1, s subcomponent
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC056903
• Gene Symbol: C1S
• Gene ID (NCBI): 716
• RRID: AB_2243610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r activates C1s so that it can, in turn, activate C2 and C4. C1s is a glycoprotein of approximately 88 kDa, which is activated by cleavage into an A chain and a B chain.

Publications

Species	Application	Title
mouse	IF	Int J Mol Sci; Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina; Authors - Anne Enzbrenner
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
human	WB	Sci Rep; Natural antibodies and CRP drive anaphylatoxin production by urate crystals.; Authors - Anne Kathrin Wessig
mouse	WB,IF	Cell Rep; Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina.; Authors - Diana Pauly
human	IHC,IF	J Transl Med; Comprehensive investigation of malignant epithelial cell-related genes in clear cell renal cell carcinoma: development of a prognostic signature and exploration of tumor microenvironment interactions; Authors - Songyang Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Asimenia (Verified Customer) (11-14-2024)

The signal of this staining was clear and with minimal background on the cells or the surrounding tissue.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: hiPSC-derived microglia

C1S Antibody Immunohistochemistry,Immunofluorescence validation (1:100 dilution) in hiPSC-derived microglia (Cat no:14554-1-AP)

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