C1QL1 Polyclonal antibody

C1QL1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 15757-1-AP

Synonyms

CRF, Complement component 1 Q subcomponent-like 1, C1QRF, C1q-related factor, C1q/TNF-related protein 14

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Validation Data Gallery

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  WB analysis using 15757-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15757-1-AP (C1QL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 15757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15757-1-AP (C1QL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 15757-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15757-1-AP (C1QL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 15757-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using C1QL1 antibody (15757-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15757-1-AP targets C1QL1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1QL1 fusion protein Ag8348
• Full Name: complement component 1, q subcomponent-like 1
• Calculated Molecular Weight: 258 aa, 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC008798
• Gene Symbol: C1QL1
• Gene ID (NCBI): 10882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1QL1 (complement component 1, q subcomponent-like 1) is one of the C1q/tumor-necrosis-factor-related protein (CTRP) family members. C1QL1 is synthesized and secreted mainly by the brain and adipose tissues. It is implicated in synapse formation or elimination in cultured hippocampal neurons in vitro.