C1qC Polyclonal antibody

• Featured Product
• KD/KO Validated

C1qC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16889-1-AP

Synonyms

C1Q C, Complement C1q C chain, C1QG

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Validation Data Gallery

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  WB analysis using 16889-1-AP

  human plasma was subjected to SDS PAGE followed by western blot with 16889-1-AP (C1qC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse serum using 16889-1-AP

  mouse serum were subjected to SDS PAGE followed by western blot with 16889-1-AP (C1qC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16889-1-AP

  rat plasma was subjected to SDS PAGE followed by western blot with 16889-1-AP (C1qC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16889-1-AP (C1QC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16889-1-AP (C1QC Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human plasma, mouse serum, rat plasma
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

16889-1-AP targets C1qC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1qC fusion protein Ag10400
• Full Name: complement component 1, q subcomponent, C chain
• Calculated Molecular Weight: 245 aa, 26 kDa
• Observed Molecular Weight: 24-26 kDa
• GenBank Accession Number: BC009016
• Gene Symbol: C1QC
• Gene ID (NCBI): 714
• RRID: AB_2878328
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

Publications

Species	Application	Title
human	WB	World J Surg Oncol; Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma; Authors - Wei Dong
human	WB	Front Genet; C1QC is a prognostic biomarker with immune-related value in kidney renal clear cell carcinoma; Authors - Wentao Yao ; KD Validated
human	IHC	J Cancer; Identification of crucial genes through WGCNA in the progression of gastric cancer; Authors - Rui Liu

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