C1QBP Recombinant antibody, PBS Only

C1QBP Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3K20

Cat no : 80490-1-PBS

Synonyms

gC1Q R, GC1QBP, GC1q R protein, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32

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Validation Data Gallery

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  WB analysis using 80490-1-RR (same clone as 80490-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80490-1-RR (C1QBP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 80490-1-RR (same clone as 80490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80490-1-RR (C1QBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 80490-1-RR (same clone as 80490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80490-1-RR (C1QBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80490-1-RR (same clone as 80490-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C1QBP antibody (80490-1-RR, Clone: 3K20 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 80490-1-RR (same clone as 80490-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human C1QBP (80490-1-RR, Clone:3K20) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Rabbit IgG (KFA002), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80490-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80490-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80490-1-RR against Human C1QBP were performed. The affinity constant is 0.776 nM.

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Product Information

80490-1-PBS targets C1QBP in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: C1QBP fusion protein Ag19773
• Full Name: complement component 1, q subcomponent binding protein
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC013731
• Gene Symbol: C1QBP
• Gene ID (NCBI): 708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).