C1QBP Monoclonal antibody, PBS Only

C1QBP Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F9B1

Cat no : 68084-1-PBS

Synonyms

gC1Q R, GC1QBP, GC1q R protein, Complement component 1 Q subcomponent-binding protein, mitochondrial, ASF/SF2-associated protein p32

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Validation Data Gallery

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  WB analysis using 68084-1-Ig (same clone as 68084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68084-1-Ig (C1QBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 68084-1-Ig (same clone as 68084-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68084-1-Ig (C1QBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68084-1-Ig (same clone as 68084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 68084-1-Ig (same clone as 68084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68084-1-Ig (same clone as 68084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68084-1-Ig (same clone as 68084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68084-1-Ig (C1QBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68084-1-Ig (same clone as 68084-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using C1QBP antibody (68084-1-Ig, Clone: 1F9B1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68084-1-Ig (same clone as 68084-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human C1QBP (68084-1-Ig, Clone:1F9B1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68084-1-PBS in a different storage buffer formulation.

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Product Information

68084-1-PBS targets C1QBP in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: C1QBP fusion protein Ag19773
• Full Name: complement component 1, q subcomponent binding protein
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC013731
• Gene Symbol: C1QBP
• Gene ID (NCBI): 708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).