C19orf52 Polyclonal antibody

• Featured Product
• KD/KO Validated

C19orf52 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 25652-1-AP

Synonyms

TIMM29, TIM29, Mitochondrial import inner membrane translocase subunit Tim29

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 25652-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25652-1-AP (C19orf52 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 25652-1-AP

  IP result of anti-C19orf52 (IP:25652-1-AP, 3ug; Detection:25652-1-AP 1:700) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 25652-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25652-1-AP (C19orf52 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 25652-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25652-1-AP (C19orf52 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below

Product Information

25652-1-AP targets C19orf52 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C19orf52 fusion protein Ag22251
• Full Name: chromosome 19 open reading frame 52
• Calculated Molecular Weight: 260 aa, 29 kDa
• Observed Molecular Weight: 29-32 kDa
• GenBank Accession Number: BC005075
• Gene Symbol: C19orf52
• Gene ID (NCBI): 90580
• RRID: AB_2880177
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Acylglycerol Kinase Maintains Metabolic State and Immune Responses of CD8+ T Cells.; Authors - Zhilin Hu
human	WB	Mol Cell; Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.; Authors - Milena Vukotic
human	WB	J Med Virol; TIM22 and TIM29 inhibit HBV replication by up-regulating SRSF1 expression; Authors - Lin Guo ; KD Validated
human	WB	J Mol Biol; Mitochondrial Oxidative Stress Induces Rapid Intermembrane Space/Matrix Translocation of Apurinic/Apyrimidinic Endonuclease 1 Protein through TIM23 Complex.; Authors - Arianna Barchiesi
human	WB	Mol Biol Cell; Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria.; Authors - Katarzyna Justyna Chojnacka
human	WB	FEBS Lett; TIM29 is a subunit of the human carrier translocase required for protein transport.; Authors - Sylvie Callegari ; KD Validated