C19orf21 Polyclonal antibody

C19orf21 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 26338-1-AP

Synonyms

C19orf21, MISP, Mitotic interactor and substrate of PLK1

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Validation Data Gallery

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  WB analysis of MCF-7 using 26338-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26338-1-AP (C19orf21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 26338-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26338-1-AP (C19orf21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 26338-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26338-1-AP (C19orf21 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 26338-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26338-1-AP (C19orf21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HepG2 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

The immunogen of 26338-1-AP is C19orf21 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C19orf21 fusion protein Ag23712
• Full Name: chromosome 19 open reading frame 21
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC052236
• Gene Symbol: C19orf21
• Gene ID (NCBI): 126353
• RRID: AB_2880482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C19orf21, also named as MISP, plays a role in mitotic spindle orientation and mitotic progression. It may be required for directed cell migration and centrosome orientation. C19orf21 regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner.

Publications

Species	Application	Title
human	WB	Front Oncol; Up-Regulated MISP Is Associated With Poor Prognosis and Immune Infiltration in Pancreatic Ductal Adenocarcinoma.; Authors - Xinyang Huang
human	WB	Cell Death Discov; Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.; Authors - Bin Zhou
mouse	WB,IF	J Vet Med Sci; Mitotic spindle positioning protein (MISP) deficiency exacerbates dextran sulfate sodium (DSS)-induced colitis in mice; Authors - Koki Hiura ; KO Validated
human	WB,IF,IP	Int J Mol Sci; Mitotic Spindle Positioning (MISP) Facilitates Colorectal Cancer Progression by Forming a Complex with Opa Interacting Protein 5 (OIP5) and Activating the JAK2-STAT3 Signaling Pathway; Authors - Koki Hiura ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size and no additional background bands were observed around the size range. Perfect!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T, HaCaT, U2OS

C19orf21 Antibody Western Blot, validation (1:1000 dilution) in 293T, HaCaT, U2OS (Cat no:26338-1-AP)