C16orf75 Polyclonal antibody

• 

  WB analysis using 30529-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30529-1-AP (C16orf75 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U-251 using 30529-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using C16orf75 antibody (30529-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

C16orf75 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30529-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

RMI2, RecQ-mediated genome instability protein 2, hRMI2, BLM-associated protein of 18 kDa, BLAP18

---

Tested Applications

• Positive WB detected in: HT-1080 cells, HeLa cells, MCF-7 cells, U-251 cells
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30529-1-AP targets C16orf75 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C16orf75 fusion protein Ag32880
• Full Name: chromosome 16 open reading frame 75
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC031016
• Gene Symbol: C16orf75
• Gene ID (NCBI): 116028
• RRID: AB_3086351
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96E14
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C16orf75 is also named as BLM-associated protein of 18 kDa (BLAP18), RMI2, hRMI2 and RecQ-mediated genome instability protein 2. C16orf75 is essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. And RMI2 is essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM (PMID: 18923082, PMID: 18923083, PMID: 27977684).