PA1 Polyclonal antibody

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  WB analysis using 20354-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20354-1-AP (PA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PA1 Polyclonal Antibody for WB, ELISA

Cat No. 20354-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C16orf53, PAXIP1 associated protein 1, PAXIP1 Associated Glutamate Rich Protein 1, PAGR1, Glutamate-rich coactivator interacting with SRC1

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20354-1-AP targets PA1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PA1 fusion protein Ag14198
• Full Name: chromosome 16 open reading frame 53
• Calculated Molecular Weight: 254 aa, 28 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC003640
• Gene Symbol: PA1
• Gene ID (NCBI): 79447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BTK6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAGR1 (PAXIP1 Associated Glutamate Rich Protein 1), also known as PA1 or C16orf53, the PA1 protein forms a complex with the histone H3K4 methyltransferases MLL3 and MLL4 and interacts with PAXIP1 (PAX Interacting Protein 1). PA1 is associated with the DNA damage response, is involved in early embryonic development, and may play a key role in male reproduction. PA1 has estrogen receptor binding activity and is involved in the endogenous positive regulation of the intracellular estrogen receptor signaling pathway.