Nesprin 3 Monoclonal antibody

Nesprin 3 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68420-1-Ig
Clone No.1D1G12

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF-P, ELISA

C14orf49, FLJ25605, Nesprin 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, C2C12 cells, PC-12 cells, rat testis tissue
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68420-1-Ig targets Nesprin 3 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Nesprin 3 fusion protein Ag26012 Predict reactive species
Full Name chromosome 14 open reading frame 49
Observed Molecular Weight110 kDa
GenBank Accession NumberBC146604
Gene Symbol Nesprin 3
Gene ID (NCBI) 161176
RRIDAB_3085135
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6ZMZ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).

Protocols

Product Specific Protocols
WB protocol for Nesprin 3 antibody 68420-1-IgDownload protocol
IF protocol for Nesprin 3 antibody 68420-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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