Nesprin 3 Monoclonal antibody

Nesprin 3 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1D1G12

Cat no : 68420-1-Ig

Synonyms

C14orf49, FLJ25605, Nesprin 3

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Validation Data Gallery

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  WB analysis using 68420-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68420-1-Ig (C14orf49 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 68420-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using Nesprin 3 antibody (68420-1-Ig, Clone: 1D1G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, MOLT-4 cells, C2C12 cells, PC-12 cells, rat testis tissue
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68420-1-Ig targets Nesprin 3 in WB, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Nesprin 3 fusion protein Ag26012
• Full Name: chromosome 14 open reading frame 49
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC146604
• Gene Symbol: Nesprin 3
• Gene ID (NCBI): 161176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nesprin 3 also known as C14orf49, SYNE3, belongs to the Nesprin family. Nesprin-3 is a type II transmembrane protein of the outer nuclear membrane and contains a C-terminal KASH domain and a series of spectrin repeats(PMID: 16330710). Nesprin-3, a protein that links intermediate filaments to the nucleus, regulates endothelial cell morphology, perinuclear cytoskeletal organization, and flow-induced cell polarization and migration. Nesprin-3 is robustly expressed in human aortic endothelial cells and localizes to the nuclear envelope(PMID: 21937718).