C-Kit Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP01171-4

  Sandwich ELISA standard curve of MP01171-4, Human CD117/c-Kit Recombinant Matched Antibody Pair - PBS only. 84259-8-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02323. 84259-6-PBS was HRP conjugated as the detection antibody. Range: 0.313-10 ng/mL.

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  Sample test of MP01171-4

  Hel 92.1.7 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human CD117/c-Kit, and measured 2.01 ng/mL.

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  Sample test of MP01171-4

  Serum of six individual healthy human donors was measured. The CD117/c-Kit concentration of detected samples was determined to be 399.22 ng/mL with a range of 257.05-736.28 ng/mL.

C-Kit {ptg:Brand} Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84259-6-PBS

Clone No.241313D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

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Product Information

84259-6-PBS targets C-Kit as part of a matched antibody pair:

MP01171-4: 84259-8-PBS capture and 84259-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• Calculated Molecular Weight: 976 aa, 110 kDa
• GenBank Accession Number: BC071593
• Gene Symbol: CD117
• Gene ID (NCBI): 3815
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10721
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.