TBXT Polyclonal antibody

TBXT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 20741-1-AP

Synonyms

Brachyury protein, Brachyury T, Brachyury-T, Protein T, T, brachyury homolog (mouse), T-box transcription factor T, TBXT, TFT

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Validation Data Gallery

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  WB analysis of mouse liver using 20741-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20741-1-AP (Brachyury-T antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20741-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20741-1-AP (Brachyury-T antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20741-1-AP targets TBXT in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: T, brachyury homolog (mouse)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: NM_003181
• Gene Symbol: T
• Gene ID (NCBI): 6862
• RRID: AB_2878730
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Brachyury T is involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. It binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). The antibody is specific to Brachyury T.