Filter: AllWBIHCFC (Intra)

WB analysis of pig skin using 67275-1-Ig (same clone as 67275-1-PBS)
pig skin tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 67275-1-Ig (same clone as 67275-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 67275-1-Ig (same clone as 67275-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
WB analysis of pig colon using 67275-1-Ig (same clone as 67275-1-PBS)
pig colon tissue were subjected to SDS PAGE followed by western blot with 67275-1-Ig (Biglycan antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human lung using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 67275-1-Ig (same clone as 67275-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67275-1-Ig (Biglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 67275-1-Ig (same clone as 67275-1-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Biglycan Monoclonal antibody (67275-1-Ig, Clone:1E3F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.
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1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Biglycan Monoclonal antibody (67275-1-Ig, Clone:1E3F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67275-1-PBS in a different storage buffer formulation.

Biglycan Monoclonal antibody, PBS Only

Biglycan Monoclonal Antibody for Indirect ELISA, IHC, WB

Cat No. 67275-1-PBS

Clone No.1E3F2

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

pig, mouse, human

Applications

Indirect ELISA, IHC, WB

Synonyms

BGN, 1E3F2, PG-S1, PGI, PG S1

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67275-1-PBS targets Biglycan in Indirect ELISA, IHC, WB applications and shows reactivity with pig, mouse, human samples.

Tested Reactivity	pig, mouse, human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	Biglycan fusion protein Ag8012 Predict reactive species
Full Name	biglycan
Calculated Molecular Weight	42 kDa
Observed Molecular Weight	48 kDa
GenBank Accession Number	BC004244
Gene Symbol	Biglycan
Gene ID (NCBI)	633
RRID	AB_2882544
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P21810
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

Biglycan is a member of the small leucine-rich proteoglycan (SLRP) family (PMID: 18463092). It consists of a core protein of 42 kDa containing leucine-rich repeats (LRRs), to which two chondroitin/dermatan sulfate side chains are covalently bound (PMID: 2212616; 10383378). The tissue-specific chondroitin- or dermatan-sulfate glycosaminoglycan chains of biglycan are attached to amino acid residues at the N-terminus of the core protein (PMID: 22821552). Biglycan is a ubiquitous component of connective tissue matrix and may act as a signaling molecule (PMID: 22821552). Upregulation of biglycan has been reported in multiple types of solid cancer (PMID: 32194659).