Tested Applications
Positive WB detected in | Jurkat cells, K-562 cells, HEK-293 cells |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83715-1-RR targets Bif-1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Bif-1 fusion protein Ag7710 Predict reactive species |
Full Name | SH3-domain GRB2-like endophilin B1 |
Calculated Molecular Weight | 41 kDa |
Observed Molecular Weight | 41 kDa |
GenBank Accession Number | BC007455 |
Gene Symbol | Bif-1 |
Gene ID (NCBI) | 51100 |
RRID | AB_3671317 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9Y371 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).
Protocols
Product Specific Protocols | |
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WB protocol for Bif-1 antibody 83715-1-RR | Download protocol |
IF protocol for Bif-1 antibody 83715-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |