Various lysates were subjected to SDS PAGE followed by western blot with 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of A549 using 66586-1-Ig
WB result of Beta Galactosidase antibody (66586-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Galactosidase transfected A549 cells.
WB result of Beta Galactosidase antibody (66586-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Galactosidase transfected A549 cells.
IHC staining of human kidney using 66586-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 66586-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 66586-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Beta Galactosidase antibody (66586-1-Ig, Clone: 4F4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
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human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.
Protocols
Product Specific Protocols
WB protocol for Beta Galactosidase antibody 66586-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of A549 using 66586-1-Ig
WB result of Beta Galactosidase antibody (66586-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Beta Galactosidase transfected A549 cells.
IHC Figures
IHC staining of human kidney using 66586-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 66586-1-Ig
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66586-1-Ig (Beta Galactosidase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 66586-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Beta Galactosidase antibody (66586-1-Ig, Clone: 4F4F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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