Beta Catenin Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Beta Catenin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17565-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 17565-1-AP (same clone as 17565-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17565-1-AP (Beta Catenin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17565-1-AP (same clone as 17565-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 17565-1-AP (same clone as 17565-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 17565-1-AP (same clone as 17565-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 17565-1-AP (same clone as 17565-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 17565-1-AP (same clone as 17565-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 17565-1-AP (same clone as 17565-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 17565-1-AP (same clone as 17565-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 17565-1-AP (same clone as 17565-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 17565-1-AP (same clone as 17565-1-PBS)

  IP result of anti-Beta Catenin (IP:17565-1-AP, 4ug; Detection:17565-1-AP 1:5000) with HEK-293 cells lysate 1920 ug. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 17565-1-AP (same clone as 17565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 17565-1-AP (same clone as 17565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17565-1-AP (same clone as 17565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17565-1-AP (same clone as 17565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 17565-1-AP (same clone as 17565-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 17565-1-AP (same clone as 17565-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 17565-1-AP (same clone as 17565-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

• 

  IF Staining of T-47D using 17565-1-AP (same clone as 17565-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 17565-1-AP (same clone as 17565-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 17565-1-AP (same clone as 17565-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Beta Catenin (17565-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

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Product Information

17565-1-PBS targets Beta Catenin in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Catenin fusion protein Ag11763
• Full Name: catenin (cadherin-associated protein), beta 1, 88kDa
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 92 kDa
• GenBank Accession Number: BC058926
• Gene Symbol: Beta Catenin
• Gene ID (NCBI): 1499
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)