Beta Arrestin 1 Recombinant antibody

Beta Arrestin 1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240974C3

Cat no : 83837-1-RR

Synonyms

ARRB1, ARR1, ARR 1, ARB2, ARB1

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Validation Data Gallery

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  WB analysis using 83837-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83837-1-RR (Beta Arrestin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83837-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83837-1-RR (Arrestin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83837-1-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 83837-1-RR (Arrestin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83837-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83837-1-RR against Human Beta Arrestin 1 were performed. The affinity constant is 0.759 nM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, RAW 264.7 cells, mouse brain tissue, rat lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83837-1-RR is Beta Arrestin 1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Beta Arrestin 1 fusion protein Ag7608
• Full Name: arrestin, beta 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-55 kDa
• GenBank Accession Number: BC003636
• Gene Symbol: Beta Arrestin 1
• Gene ID (NCBI): 408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).