CoraLite® Plus 647-conjugated Beta Actin Recombinant antibody

Beta Actin Uni-rAb^TM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, canine

Applications

IF/ICC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

4H1

Cat no : CL647-81115

Synonyms

Actin, ACTB, bate actin, β actin, Actin, cytoplasmic 1

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Validation Data Gallery

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  IF Staining of MDCK using CL647-81115

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using CoraLite® Plus 647 Beta Actin antibody (CL647-81115, Clone: 4H1 ) at dilution of 1:500.

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Tested Applications

• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-81115 targets Beta Actin in IF/ICC applications and shows reactivity with human, mouse, rat, pig, canine samples.

• Tested Reactivity: human, mouse, rat, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Beta Actin fusion protein Ag14521
• Full Name: actin, beta
• Calculated Molecular Weight: 375 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC002409
• Gene Symbol: Beta Actin
• Gene ID (NCBI): 60
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )